Correlation between cryo-transmission electron microscope (TEM) images and the crystal structure. (l) TEM image showing three colliding clusters. The scale bar is 10 nm. (r) Relative positions of molecules derived from the X-ray diffraction crystal structure are overlaid (brown) on the TEM image. A twinning plane is shown (green line).Credit: Weizmann Institute of Science
Crystallization is a very basic chemical process: schoolchildren can witness it with their own eyes. But scientists had not been able to observe this process on the molecular level — that is, the instant in which molecules overcome their tendencies to float individually in a liquid solution and take their place in the rigid lattice of a solid crystal structure. Now, researchers at the Weizmann Institute of Science have, for the first time, directly observed the process of crystallization on the molecular level, validating some recent theories about crystallization as well as showing that if one knows how the crystal starts growing, one can predict the end structure. This work was published in Nature Chemistry.
The research took place in the lab of Prof. Ronny Neumann of the Weizmann Institute’s Department of Organic Chemistry. Prof. Neumann explains that in order to bond to one another, the molecules must overcome an energy barrier: “The prevalent theory had been that chance contacts between molecules leads to bonding, eventually creating small clusters that become nuclei for larger crystals to grow. But the molecules, which move randomly in solution, must be aligned properly to crystalize. In recent years researchers have begun to think that this process might present too high an energy barrier.”
Theories proposed in the past few decades suggest that if the molecules were to congregate together in a so-called “dense phase,” in which they aggregate into a sardine-like state — close together but unorganized — and then crystallize from this state, the energy barrier would be lower.
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To test the theories, Prof. Neumann and PhD student Roy Schreiber created large, rigid molecules and froze them in place in solution. They then placed the frozen solution under an electron microscope beam that warmed up the mixture just enough to allow some movement — and, thus, interactions between the molecules. Adjusting the makeup of the solution by adding different ions enabled the scientists to produce crystallization with and without dense phases; aided by Drs. Lothar Houben and Sharon Wolf of the Electron Microscopy Unit, they were able to observe, for the first time, dense phases forming and subsequently transforming into crystal nuclei.
While both states yielded crystals, the experimental results showed that when dense phases form, the energy barrier to formation of an orderly, crystalline arrangement of molecules is lower — just as the theory predicted.
The scientists also found that the growth arising from dense phases results in larger, more stable crystal nuclei. In addition, they discovered that the arrangement of molecules in fully grown crystals, which they determined by X-ray crystallography with the aid of Dr. Gregory Leitus of Chemical Research Support, was in good agreement with that in the small clusters of just a few molecules in the original nuclei. “This means that the forces and factors that determine the process are constant throughout the growth of the crystal,” says Prof. Neumann.
“We have really observed an elementary event in the world of chemistry,” Prof. Neumann states. “The findings are also leading us into new inquiries in this area, looking at the effects and significance of dense phases on chemical reactivity.”
Also participating in this research was a group led by Prof. Josep Poblet, Dr. Jorge Carbó, and PhD student Zhong-Ling Lang of the University of Rovira i Virgili, Tarragona, Spain, who assisted in the calculation of the motion of molecules in solution.
Source: Weizmann Institute of Science
Roy E. Schreiber, Lothar Houben, Sharon G. Wolf, Gregory Leitus, Zhong-Ling Lang, Jorge J. Carbó, Josep M. Poblet, Ronny Neumann. Real-time molecular scale observation of crystal formation. Nature Chemistry, 2016; DOI: 10.1038/nchem.2675