ALS study reveals role of RNA-binding proteins



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als-study-reveals-role-of-rna-binding-proteins

Donated human skin samples were used to produce first induced pluripotent stem cells (iPSCs), then ultimately the healthy, donor-specific motor neurons shown here.Credit: UC San Diego Health

Although only 10 percent of amyotrophic lateral sclerosis (ALS) cases are hereditary, a significant number of them are caused by mutations that affect proteins that bind RNA, a type of genetic material. University of California San Diego School of Medicine researchers studied several ALS cases with a mutation in a RNA-binding protein known as hnRNP A2/B1. In the study, published October 20 by Neuron, they describe how damage to this protein contributes to ALS by scrambling crucial cellular messaging systems.


“Our findings are a significant step forward in validating RNA-based therapy as a treatment for ALS,” said senior author Gene Yeo, PhD, professor of cellular and molecular medicine at UC San Diego School of Medicine.

ALS, also known as Lou Gehrig’s disease, is a devastating neurological condition affecting more than 20,000 Americans. The disease greatly diminishes patients’ quality of life and is terminal. ALS affects a special kind of nerve cell called a motor neuron. These motor neurons enable us to move our bodies. Currently, there are no effective treatments for ALS, largely due to poor understanding of how the disease initiates and progresses at the molecular level.

Yeo’s team studies RNA-binding proteins and their ability to control how, when and if cells make certain proteins. To unravel the role RNA-binding proteins play in ALS, Yeo’s team gathered skin cells from four patients with the disease — three with mutations in the hnRNP A2/B1 gene, one with a mutation in a different gene — and two healthy volunteers as controls. The researchers coaxed these skin cells into becoming a special kind of stem cell called induced pluripotent stem cells (iPSCs) and ultimately turned these patient-specific stem cells into motor neurons. This technique provided them with personalized models of each patient’s disease, in a laboratory dish, where it’s easy to do experiments.

To determine the effects of the mutant hnRNP A2/B1 proteins in these samples, the researchers then measured the activity of thousands of genes in each of the ALS and healthy motor neuron samples. In the ALS patient samples, Yeo and team found that the hnRNP A2/B1 mutation these patients had didn’t merely disable the protein. Instead, the mutation gave the protein new toxic properties that scrambled RNA processing, and ultimately led to the death of motor neurons.

Yeo said these findings may have important implications for their collaborators and others who are developing therapeutics that aim to treat disease by targeting RNA.

“These RNA-targeting therapies can eliminate toxic proteins and treat disease,” said first author Fernando Martinez, a graduate student in Yeo’s laboratory. “But this strategy is only viable if the proteins have gained new toxic functions through mutation, as we found here for hnRNP A2/B1 in these ALS cases.”

Environmental factors are thought to contribute to ALS, increasing the chances a person with a genetic predisposition will get the disease. To mimic this scenario, Martinez compared how motor neurons from ALS patients and healthy individuals respond to stress. In the patient-specific ALS motor neurons under stress, they found more hnRNP A2/B1 proteins clumped in a part of the cells called stress granules, as compared to healthy cells.

“Drugs that lower the exaggerated reaction to stressors that we saw in motor neurons from ALS patients may also prove a powerful therapeutic strategy,” Yeo said. “It is likely that multiple, multi-directional shots at goal will be needed to treat this debilitating disease.”

 


Source: University of California San Diego Health Sciences

Research Reference:

Fernando J. Martinez, Gabriel A. Pratt, Eric L. Van Nostrand, Ranjan Batra, Stephanie C. Huelga, Katannya Kapeli, Peter Freese, Seung J. Chun, Karen Ling, Chelsea Gelboin-Burkhart, Layla Fijany, Harrison C. Wang, Julia K. Nussbacher, Sara M. Broski, Hong Joo Kim, Rea Lardelli, Balaji Sundararaman, John P. Donohue, Ashkan Javaherian, Jens Lykke-Andersen, Steven Finkbeiner, Frank Bennett, Manuel Ares, Christopher B. Burge, J. Paul Taylor, Frank Rigo, Gene W. Yeo. Protein-RNA Networks Regulated by Normal and ALS-Associated Mutant HNRNPA2B1 in the Nervous System. Neuron, 2016; DOI:10.1016/j.neuron.2016.09.050

Posted in Health & Medicine on October 20, 2016